Molecular Detection and Identification for Mycosis -fungal DNA extraction method-
نویسندگان
چکیده
منابع مشابه
Molecular approaches for detection and identification of foodborne pathogens
Foodborne pathogens comprise microorganisms such as viruses, bacteria and parasites that can be transmitted by food and affect public health worldwide. The most common viruses transmitted via food are hepatitis A virus and Norwalk-like caliciviruses. Also, the most common bacteria involved in foodborne illnesses are Campylobacter jejuni, Clostridium perfringens, Salmonella spp, Escherichia...
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An improved protocol for the isolation of high-quality DNA from fungi is described. The method involves inactivating proteins by SDS/proteinase K and precipitating polysaccharides in the presence of high salt. Further purification is based on differential solubility of DNA and high molecular weight polysaccharides in aqueous media. The procedure does not use the toxic and potentially hazardous ...
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ژورنال
عنوان ژورنال: Medical Mycology Journal
سال: 2013
ISSN: 2185-6486,2186-165X
DOI: 10.3314/mmj.54.329